In story of shippuden 456. Read manga chapter in eye is slowly becoming dark spoiler story from
shippuden blog and story by xiraiya.
Detail chapter shippuden. From spoiler say: "Problem is, we just don't know for sure, it's possible since Kakashi knew about Mangekyou and what it was that he knew of it's effect, however that comment does suggest his eye is slowly becoming dark, though some sharingan take longer than others it seems, considering sasuke's supposidly have greater potential and are already going blind then one can suggest that. The greater the sharingan, the shorter the lifespam it has one activating MS, Itachi had his for nearly a good 10 years or so before going blind, Sasuke has had his not long at all and he's already suffering the effects. We can also see this with Madara and his brother they both activated MS at around the same time, however Madara went blind first while his brother apparently hadn't suffered any sight damage yet."
Present shippuden manga 456. Retrace at
Shippuden 456 Opponents with A Sharingan Feet
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